diff --git a/modules/top-level/output.nix b/modules/top-level/output.nix
index 06b8d90b..93e0aed1 100644
--- a/modules/top-level/output.nix
+++ b/modules/top-level/output.nix
@@ -95,6 +95,13 @@ in
         })
       ) allPlugins;
 
+      # Python3 dependencies from all plugins
+      python3Dependencies =
+        let
+          deps = map (p: p.python3Dependencies or (_: [ ])) allPluginsOverrided;
+        in
+        ps: builtins.concatMap (f: f ps) deps;
+
       # Combine all plugins into a single pack
       pluginPack = pkgs.vimUtils.toVimPlugin (
         pkgs.buildEnv {
@@ -106,6 +113,9 @@ in
             find $out -type d -empty -delete
             runHook preFixup
           '';
+          passthru = {
+            inherit python3Dependencies;
+          };
         }
       );
 
diff --git a/tests/test-sources/modules/performance/combine-plugins.nix b/tests/test-sources/modules/performance/combine-plugins.nix
index 8a084923..c9bec0d6 100644
--- a/tests/test-sources/modules/performance/combine-plugins.nix
+++ b/tests/test-sources/modules/performance/combine-plugins.nix
@@ -102,4 +102,31 @@
         }
       ];
     };
+
+  # Test that plugin python3 dependencies are handled
+  python-dependencies.module =
+    { config, ... }:
+    {
+      performance.combinePlugins.enable = true;
+      extraPlugins = with pkgs.vimPlugins; [
+        # No python3 dependencies
+        plenary-nvim
+        # Duplicate python3 dependencies
+        (nvim-lspconfig.overrideAttrs { passthru.python3Dependencies = ps: [ ps.pyyaml ]; })
+        (nvim-treesitter.overrideAttrs { passthru.python3Dependencies = ps: [ ps.pyyaml ]; })
+        # Another python3 dependency
+        (nvim-cmp.overrideAttrs { passthru.python3Dependencies = ps: [ ps.requests ]; })
+      ];
+      extraConfigLuaPost = ''
+        -- Python modules are importable
+        vim.cmd.py3("import yaml")
+        vim.cmd.py3("import requests")
+      '';
+      assertions = [
+        {
+          assertion = builtins.length config.finalPackage.packpathDirs.myNeovimPackages.start == 1;
+          message = "More than one plugin is defined in packpathDirs.";
+        }
+      ];
+    };
 }